Rajashree Srinivasa*, N. Siddiqui** and Renu Mishra*

*Sri Sathya Sai College for Women, Bhopal-462024 (India) **Govt. Gitanjali P.G. College, Bhopal-462038 (India) Email: rajirocking@gmail.com

ABSTRACT

Gloriosa superba is frequently used in traditional medicine system for treatment of several diseases including infectious ones. Several active phytochemicals are present in this plant and they show remarkable effect on pathogenic bacteria. In vitro propogation was carried out for direct regeneration of clone plants with same genotype. Optimized hormonal combination of 5.0 mg/l BAP+1.0 mg/l NAA showed 88% bud initiation and multiplication in Gloroisa superba was maintained as control medium. Various supplements like coconut water, GA3, ABA, and Biotin were added to enhance shoot multiplication in tuber sprouts of Gloriosa superba. With addition of 15% coconut water (cw) there was enhancement in shoot multiplication (14.6 ±1.1cm), length of shoot was 5.6 ±0.6 cm and days taken were twenty two. Coconut water improved both the quality and development of shoots in in-vitro cultured Gloriosa superba. The other additives such as GA3, Biotin and ABA had less or no supportive effect on multiplication of shoots in Gloriosa superba explant.

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