Effect of Host substrate (Vicia Faba L.) on the growth of Sclerotium Rolfsii Sacc.
Irungbam Jamuna Devi
Department of Botany, Nambol L. Sanoi College, Nambol-795134 (India)
ABSTRACT
Sclerotium rolfsii Sacc. [teleomorph Athelia rolfsii (Curzi) C.C. Tu & Kimbr.] is a soil borne fungus which is particularly severe on leguminous crops. Broad bean (Vicia faba L.) is one of the major pulse crops in Manipur using both as vegetable and pulse grain. Wilt disease which is a complex disease caused by Sclerotium rolfsii, causes great reduction in the annual production of the broad bean. As the legumes are the important source of vegetable protein, protection of these crops from diseases is also veryimportant. The effect of substrate extract on the radial growth of S. rolfsii was performed using Czapek-dox agar medium amended with leaf, stem and root extracts of Vicia faba at concentrations of 1, 10, 20 and 50% (v/v). It was observed that the leaf extract (1% and 10% concentration) was stimulatory on the radial growth of S. rolfsii during early incubation period (12-24 hrs.) and inhibitory effect was observed during late incubation period (36-60 hrs.). Highest concentration 50% of leaf extract gave the highest inhibitory effect was found during 36 hrs. incubation period. It was also observed that both stem and root extract of Vicia faba gave significant inhibition on the radial growth of S. rolfsii.