In vitro regeneration of plantlets of Albizzia lebbeck (L.) Benth. through direct shoot bud development from different explants on MS solid and liquid media
Sharmistha Maity
Department of Botany, Krishnagar Government College, Krishnagar, Nadia-741101, West Bengal (India) E-mail address: sharmistha_maity@ rediffmail.com; Mobile No. - 9832223933
ABSTRACT
Albizzia lebbeck is a deciduous tree of moderate to large size. Despite its fuel yielding value, the plant is reported to have antiasthmatic, anti-inflammatory, antifertility and anti-diarrhoeal properties and an important source of chemicals of D-catechin, b-Sitosterol, Albiziahexoside etc., which are effective as antiseptic, anti-dysenteric, anti-tubercular and used in bronchitis, leprosy, paralysis, helminthes infection etc. Conventionally, Albizzia lebbeck is propagated from seed however, prolonged dormancy, rapid loss of viability, and low germination rates limit natural propagation. Therefore, to meet the ever increasing demand of planting material, an in vitro regeneration protocol of this plant species is very important. Cotyledons, hypocotyls and leaves were used as explants for in vitro plant regeneration of Albizzia lebbeck through direct shoot bud development on MS solid and liquid media supplemented with various concentrations of 2-ip in combination with coconut water. A very high number of shoot buds were regenerated both in liquid and semi solid cultures in all explants. However, liquid media proved its supremacy over semi solid culture. Abbreviations : 2iP- N6-(Δ2-isopentenyl) adenine, ANOVA- analysis of variance, BAP- N6- benzylaminopurine, CW- Coconut water, DMRT- Duncan’s multiple range test, Kn- Kinetin, MS- Murashige and Skoog medium, NAA- α-Napthalene acetic acid, PGR- Plant growth regulator, SE- standard error.