Effect of Growth regulators on in vitro shooting and rooting of Solanum xanthocarpum Schradt. & Wendl. and its Micropropagation for future medicinal applications
Mamta1 and Rekha Pimpalgaonkar2
1Research Scholar, Department of Botany, Govt. College of Science Raipur-492001 Pt. Ravishankar Shukla University, Chattisgarh (India) Email- mamta393@gmail.com Contact Number-07441157386 2Professor and Head of Botany Department, Govt. College of Science Raipur-492001 Pt. Ravishankar Shukla University, Chattisgarh (India)
ABSTRACT
The medicinal plant Solanum xanthocarpum Schradt. & Wendl. belong to family Solanaceae, which is a perennial, prostrate, ramose and prickly plant with green and white fruits but yellowish at maturity. The fruits are known for several medicinal uses like anthelmintic, anti pyretic, laxative, anti-inflammatory and anti-asthmatic. This present investigation aimed at developing rapid micro propagation protocol, which can be used for conservation and mass multiplication of valuable medicinal plant of Solanum xanthocarpum to meet our pharmaceutical demand and its conservation. Optimization was made to evolove a standardized in vitro technology to conserve, as well as mass propagate this valuable medicinal herb in maximum number. A concentration of 1mg/l benzylaminopurine (BAP) showed highest shoot length, whereas maximum number of shoots per explants were obtained at 3mg/l concentration of BAP. Callus formation initiated at 5mg/l to 8mg/l of BAP. Shoot multiplication followed by subculturing which were performed in every 20 days. Rooting was optimized with different concentrations (0.25mg/l, 05mg/l, 1.0mg/l and 2.0mg/l) of auxin i.e. indole- 3-butyric acid (IBA). Highest root length and root numbers were obtained at 1.0mg/l(IBA). Primary hardening was done at temperature 25-30oC, humidity 80-90. 70% of survivability was obtained after primary hardening. After secondary hardening 90% survivability were obtained in shade house. After secondary hardening plants were finally transferred into field where 100% survivability was obtained. This standardized micropropagation procedure could be useful for mass multiplication of superior plant material for field cultivation.