Title- Microbiology Evaluation of nutritional parameters for the enhanced production of carotenoid pigment by Planococcus maritius using one factor at- a- time methodology
Varsha M. Chaudhari
Department of Microbiology, PSGVPM’s SIP Arts, GBPatel Science and STSKVS’ Commerce College, Shahada-425 409 (India) Author for Correspondence email : varsharaj2913@gmail.com
ABSTRACT
The purpose of the present research work was to determine the influence of various nutritional parameters on the biomass and pigment production in Planococcus maritimus. A novel strain of Planococcus maritimus was found to produce an intense orange carotenoid pigment. This study revealed that among different physical parameters the maximum growth and pigment production in Planococcus was achieved when inoculated with 3% inoculum at 37°C, pH 7 with shaking at 120-150 rpm for 48 hours. Among the various carbon and nitrogen sources studied, maximum biomass and pigment production rate was found with sugar sucrose, yeast extract as the inorganic and ammonium per sulphate among the organic nitrogen source. Among the various metal ions MgSO4 and CaCl2, while K2HPO4 proved to be the best phosphate source for biomass and pigment production in isolate. When shake flask experiment containing optimized media was studied it revealed that two fold elevation in pigment production was achieved under optimum nutritional parameters.