Nikhat Parween*, Amber Jabeen and Birendra Prasad

Microbial & Molecular Genetics Lab., Department of Botany, Patna University, Patna-800005 (India) Department of Biochemistry, Patna University, Patna-800005 (India)


Eugenol, is a major volatile constituent of clove essential oil and active component of many medicinal plants. It has been reported as a potential drug to encompass life threatening indispositions including hyperglycemia, neural disorders and cancer. Due to oil characteristic of EUG, we intend to evaluate its effect on the lipid metabolism using the Caenorhabditis elegans animal model. Worms (N2, wild type) were exposed to different concentrations of eugenol (1, 10 and 50μM). Lipid accumulation was determined quantitatively by colorimetry using sulpho-phospho-vanillin reagent and qualitative analysis was carried by staining the nematodes with Sudan black B and Oil Red O. The graph was statistically analyzed using one-way ANOVA and t-test using Graphpad Prism 8.2.0. Eugenol dose dependently reduced overall lipid accumulation in C. elegans (N2, wild type). Eugenol (50μM) significantly reduced lipid accumulation by 55.03%. We further confirmed that lipid reduction up on treatment in tub-1 mutant (RB1600) and daf-16 mutant was less than that in wild type (N2) C. elegans. Overall results indicate that lipid ameliorating effect of eugenol supplementation was independent of tub- 1 and daf-16 in C. elegans. The present work demonstrated eugenol as a potential compound which ameliorates lipid accumulation and exerts its effect independent of tub-1 and daf-16 in C. elegans. So, it can be assumed that eugenol can be beneficial to humans as well, albeit further research is necessary before declaring it for human consumption.

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