Investigation on the toxic effects of toilet cleaner Domex on the Fingerlings of Puntius goninotus (Bleeker, 1850)
Shashikanth H. Majagi* and D. S. Somashekar
Department of Zoology* Vijaynagar Sri Krishnadevaraya University, Ballari-583105 Karnataka (India) P G studies in Zoology IDSG Government First Grade College, Chikkmagalur-577102 (India) smajgi@rediffmail.com
ABSTRACT
Toxicity of toilet cleaner Domex was assessed on fingerlings of fish Puntius goninotus using static toxicity method. Biochemical studies were carried out in fishes exposed to sub lethal concentration (0.03ml/l of 24 h LC50 value).The glycogen, glucose and protein contents of the tissues Viz., muscle and liver were estimated in toilet cleaner Domex exposure and control fishes. The glycogen [(0.10mg/g) in Muscle and (0.25mg/g) in gills] and protein [(0.31mg/g) in Muscle and (0.15mg/g) in gills] levels were found to be depleted in the tissues exposure to sub lethal concentration over the control [0.41 mg/g and 0.16 mg/g respectively]. Whereas the glucose level [(1.31mg/g) in Muscle and (2.32 mg/g) in gills] in the tissue showed an increase on Domex exposure fishes in comparison with the control fishes [(0.13mg/g) in Muscle and (0.08 mg/g) in gills]. The depletion of glycogen level on Domex exposure may be due to stress condition and increased metabolism. The increased in the glucose level on Domexexposure suggests that animal mobilizes the reserve carbohydrate to meets its energy needs. Hence the animal enters in to hyperglycemic condition under stress. Further depletion in total protein content may be the augmented proteolysis and possible utilization of their product for metabolic process. The behavioral changes recorded during the exposure showed decrease in opercular movement, loss of reflex, increase in erotic swimming, increase in mucous secretion all over the body and irregular swimming activity were noticed.