Study on Physiological and Nutritional Factors of Fungi isolated from External Ear Canal
Amrin Khan* and Sudhir Kumar Jain
1,2School of Studies in Microbiology, Vikram University, Ujjain-456010 (India) amrink303@gmail.com, sudhirkjain1@rediffmail.com *Corresponding author: Amrin Khan
ABSTRACT
The present study aimed to examine the nutritional requirements, temperature, and pH of fungi. The influence of culture medium, various ranges of temperature, and pH was analyzed on the mycelial growth of fungi. The test fungi isolated from Otomycotic patients. Three test fungi Aspergillus niger, Aspergillus flavus, and Candida were cultured on six different types of culture media including Sabouraud’s dextrose broth (SDB), Potato dextrose broth (PDB), Czapek’s Dox broth (CDB), Malt Extract broth (MEB), Tryptone soy broth (TSB) and Littman bile broth base (LBB). The fungal growth and sporulation were analyzed at various temperatures (20ºC, 25ºC, 30ºC, 35ºC, 40ºC, and 45ºC) and pH values (4, 5, 6, 7, 8, and 9). In the present study, Sabouraud’s dextrose broth is the most suitable broth medium for all three test fungi, Aspergillus niger (380 mg dry mycelial weight), Aspergillus flavus (308 mg dry mycelial weight), and Candida (164 mg dry mycelial weight). The optimum temperature range is 25-35ºC and the pH range is 5-7. The fungal isolates grow best at 30ºC, Aspergillus niger (329 mg dry mycelium weight), Aspergillus flavus (316 mg dry mycelium weight), and Candida (148 mg dry mycelium weight). The maximum fungal growth observed at pH 7, Aspergillus niger (325 mg dry mycelium weight), Aspergillus flavus (311 mg dry mycelium weight), and Candida (128 mg dry mycelium weight). The present study highlights the suitable nutritional media, temperature, and pH is important for enhancement in the growth and sporulation of Otomycotic fungi. This study may help to understand the growth characteristics of Otomycotic fungi.