Biochemical changes in the fermentation broth during L-glutamic acid production by a mutant Corynebacterium glutamicum X680
1*Subhadeep Ganguly and 2*Smaranika Pattnayak
1Department of Physiology, Vidyasagar College, 39 Sankar Ghosh Lane, Kolkata-700006 (India) 2Department of Biotechnology and Bioinformatics, Medical Biotechnology Laboratory, Sambalpur University, Jyoti Vihar, Burla-768019 (India) *Address for correspondence: res_biol@rediffmail.com/smaranika2010@gmail.com
ABSTRACT
The present study was undertaken to investigate the biochemical changes in the fermentation broth during submerged fermentation of L-glutamic acid using a biotin auxotroph Corynebacterium glutamicum X680. Dry cell weight increased with the concomitant rise of L-glutamic acid production along with the accumulation of α-ketoglutarate, pyruvate and succinate in the fermentation broth. residual sugar content decreased gradually. But the production decreased after 72h of incubation period. The pH of the medium decreased gradually with time due to accumulation of metabolic acids. The amino nitrogen increased up to 72h of incubation as an indicator of the product (L-glutamic acid) formation. Cell nitrogen and ammonical nitrogen also increased gradually throughout the fermentation period. This study focused on the pattern of byproduct formation and overall metabolic status of the microorganism during Lglutamic acid production.