Polyphasic Characterisation and Phylogenetic Affiliation of Beta Glucanase Producing Actinomycetes Strains TBG-MR17 and TBG-AL13, Isolated from Western Ghats Soil, Kerala, India
Lekshmi K. Edison1, Ragitha V. M. 1 and N. S. Pradeep2*
1Microbiology Division, KSCSTE-Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram-695 562 (India) 2*KSCSTE-Malabar Botanical Garden and Institute of Plant Sciences, Kozhikode-673014 (India) *Correspondance: drnspradeep@gmail.com, Tel: +91 9446093865
ABSTRACT
Soil is a natural reserve encompassing the diversity of microorganisms, with amazing production of useful metabolites. Over the past few decades, an enormous number of actinomycetes species have been isolated from soil samples. The isolation and taxonomical description of actinomycetes with the novel metabolites production from under-exploited or unexplored environments is tremendously crucial, which unlocks exhilarating avenues in biotechnology research. The polyphasic taxonomic approach exploiting morphological, cultural, biochemical, physiological, and molecular characteristics, assist the taxonomists to develop universal eloquent actinomycetes taxonomic identification system. This approach effectively identifies actinomycetes isolates from the genus level and fairly extends up to the species level. In this study, the cumulative result of polyphasic characterization elucidates the taxonomic position of efficient β-glucanase producing Western Ghats actinomycetes isolates TBG-MR17 and TBG-AL13 respectively as Streptomyces althioticus TBG-MR17 and Streptomyces cinereoruber sub sp. cinereoruber TBG-AL13.