Antibacterial and mosquitocidal potential of Fusarium annulatum (Entomopathogenic Fungi) from Agricultural soil
1B. Sowbakkiyalakshmi and *K. Kolanjinathan
Department of Microbiology, Faculty of Science, Annamalai University, Annamalai Nagar, Chidambaram-608002 (India) * Corresponding author mail id: drkolanji@gmail.com
ABSTRACT
The current study focused on the secondary metabolites of the fungus Fusarium sp. against pathogenic bacteria and vector mosquitoes. The soil samples were collected from 50 different areas of agricultural fields in Cuddalore district. The possessed fungus was isolated and identified using the appropriate methods, and from the categorised isolates, the highest secondary metabolites produced by potential fungi were screened. The highest potential secondary metabolites produced by Fusarium annulatum was identified by molecular sequencing method. Then secondary metabolites were produced from mass cultivated fungi and separated by the ethyl acetated solvent technique. In the present study, antibacterial and mosquitocidal activities were performed by collected secondary metabolites against three different mosquito larvae and selected pathogenic bacteria. At a concentration of 150 mg/ml, the extract was discovered to be potentially effective against Staphylococcus aureus (19 mm), Streptococcus pyogenes (18 mm), Streptococcus pneumonia (17mm), Escherichia coli (19 mm), Enterococcus fecalis (17 mm), Pseudomonas aeruginosa (17 mm) and Proteus sp (17 mm). The mosquitocidal activity done for the 3 common vectors were Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus, LC50 and LC90 values achieved by the Fungi extract were 38.81 μg/ml and 215.17μg/ml for Ae. aegypti larvae when compared to An. stephensi and Cx. quinquefasciatus larvae was relatively more resistance to the fungal extract. The findings support the notion that fungi secondary metabolites are a suitable source of biologically active compounds for drug discovery and the development of biopesticides since they demonstrate that the Fusarium annulatum fungal crude extract exhibits antibacterial and mosquitocidal activities.