Rapid and large scale micropropagation of true to type clone of Mentha arvensis Linn. (Lamiaceae) -A valuable medicinal plant
Susanta Kumar Maity*, Anadi Kumar Kundu and Brij Kumar Tiwary
Department of Botany, Chandernagore Govt. College, Chandernagore, Hooghly, West Bengal (India)
ABSTRACT
Method for in vitro micropropagation of Mentha arvensis Linn. by axillary bud proliferation were investigated by optimizing the concentrations and combinations of different phytohormones in MS medium. Axillary bud explants could be stimulated to form multiple shoots on MS medium augmented with BAP and AdS. The best proliferation (8.81 shoots per explant) was achieved on BAP (1.0 mg/l). Shoots could be easily rooted on MS medium containing 1 mg/l IBA. Plantlets were successfully acclimatized in green house and field conditions. No detectable differences were recorded between the donor and in vitro generated plants with regard to biochemical and cytological parameters