In vitro plant regeneration of Bauhinia variegata Linn. through direct and indirect shoot bud development from different explants on MS solid and liquid media
Pintu Banerjee
Department of Botany, Krishnagar Government College, Krishnagar, Nadia-741101, West Bengal (India) E-mail address: pintu.banerjee@ rediffmail.com; Mobile No. - 9832129050
ABSTRACT
The conventional methods of propagation of Bauhinia variegata Linn., sexual as well as vegetative, are impeded with many problems that restrict their multiplication on a large scale. A fairly long time gap between pod formation and maturation hampers adequate supply of seeds in natural conditions. Therefore, to meet the ever increasing demand of planting material, an in vitro regeneration protocol of this plant species is very important. Cotyledons, hypocotyls and leaves were used as explants for in vitro plant regeneration of Bauhinia variegata through direct and indirect shoot bud development on MS solid and liquid media supplemented with various concentrations of cytokinin and auxin in combination or singly with coconut water. A very high number of shoot buds were regenerated both in liquid and semi solid cultures in all explants. However, liquid media proved its supremacy over semi solid culture.