The use of indigo carmine for the rapid determination of the germinative capacity of Cassia fistula Linn. seeds
Mukta Shrivastava
Department of Botany Govt. M.L.B. Girls College, Bhopal M.P. (India)
ABSTRACT
Viable seed is one which is capable of germinating under the proper circumstances. Seed viability of Cassia fistula was assessed with 1% indigo carmine staining which stained the dead tissue. On the basis of staining pattern (embryo + cotyledon) seeds were classified into various staining categories for the detection of viability. An attempt was also made to classify seed vigour on the basis of indigo carmine staining. It was found that indigo carmine test showed viability nearest to the laboratory gemination, easily soluble in water and takes less time to determine the viability.