Toxicity of Dichloroacetic Acid (DCA) in the testis of Albino rats
R. Sen, P. Ahirwar, S.A. Shaffi1, A. Chauhan2 and A.H. Khan3
1Department of Zoology, Regional Institute of Education (NCERT), Shyamla Hills, Bhopal-462001 (India) 2S.R.F., JICA Research Project, Department of Plant Pathology, R.A.K. College of Agriculture, Sehore-466001 (India) 3Department of Zoology, Saifia Science College, Bhopal-462001 (India) E-mail:- renusenphd82@gmail.com, abhibci@gmail.com, priyanka.ahirwar1984@yahoo.in
ABSTRACT
Dichlroacetic acid (DCA) is a haloacetic acid compound. It is a disinfection by-product (DBPs) and also used as a therapeutic agent. It is also know as dichloroacetate and dichloroethanoic acid. Male albino rats were orally administered with 125mg/kg-body weight of Dichloroacetate for 30 days, 60 days and 90 days. The animals were sacrificed and testis was quickly dissected out and fix in 10% formalin for rutine histological techniques. The 30 days treated rats, histology showed minimum impact on the process of spermatogenesis and few sperms were present in lumen (LU), disorganization of the epithelium (EP) and leydig cell (LC) hypertrophy at 60 days and the germinal epithelium (GE) degeneration and the seminiferous tubules filled with giant cell (GC) at 90 days. These findings indicate that DCA is reproductive toxicant and cause deleterious effect on cells of germinal epithelium and leydig cells of the testis and its probable adverse effects on spermatogenesis which may cause male infertility is discussed.