Preparation of Ag nanoparticles by biological method
Hamid Reza Ghorbani
Department of Chemical Engineering,Qaemshahr Branch, Islamic Azad University, Qaemshahr (Iran) *Corresponding author: hamidghorbani6@gmail.com
ABSTRACT
Development of reliable and eco-friendly processes for synthesis of metallic nanoparticles is an important step in the field of application of nanotechnology. One of the options to achieve this objective is to use natural factories such as biological systems. In this study, we have investigated the synthesis of nanoparticles of silver by reduction of aqueous Ag+ ions with the culture supernatant of Klebsiella pneumonia. UV–visible spectrum of the aqueous medium containing silver ion showed a peak at 425 nm corresponding to the plasmon absorbance of silver nanoparticles. The DLS analysis showed silver nanoparticles with size of 85±25 nm. In addition, it was used from Scanning electron microscopy (SEM).The process of reduction is extracellular, which makes it an easier method for the synthesis of silver nanoparticles.