In vitro propagation of Psoralea corylifolia L. -An important Endangered medicinal plant
Nelofar Gulam Nabi1* and Mukta Shrivastava2*
Department of Botany, Govt. MLB Girls P.G. College Bhopal-462 003 (India) 1*nelofarnabi2@gmail.com 2* mukta.shrivastava07@gmail.com
ABSTRACT
Psoralea corylifolia L. belongs to the family Fabaceae. Its natural population has declined very fast due to indiscriminate and illegal collections and destruction of its habitats and as a result it is included in the endangered list of plants. It’s in vitro protocol techniques are currently unavailable to help growers to meet the demand of the plant for cultivation and pharmaceutical industry. The present study deals with rapid and efficient protocol development for in vitro propagation of Shoot induction on Murashige and Skoog medium supplemented with various auxins and cytokinins individually and in various combinations has been achieved by using axillary and apical meristems. MS medium fortified with 0.5- 2.0 mg/l BAP and 0.2mg/l NAA was found to be effective individually. The medium with 0.5mg/l BAP + 0.2 mg/l NAA responded better as compared to other combinations. 3-6 shoots having 2-3cm length has been initiated from axillary meristem were excised and further used for shoot multiplication on MS fortified with high concentration of growth hormones to produce shoots.