Spilanthes acmella Murr. An important endangered medicinal plant and its conservation through Tissue culture techniques
Nelofar Gulam Nabi and Mukta Shrivastava
Department of Botany Govt., MLB Girls P.G. College Bhopal-462003 (India) nelofarnabi2@gmail.com ; mukta.shrivastava07@gmail.com.
ABSTRACT
Spilanthes acmella Murr. belongs to the Genus: Spilanthes, Family: Asteraceae (Compositae). Its natural population has declined very fast due to indiscriminate and illegal collections and destruction of its habitats as a result it is included in the endangered list of plants. It’s in vitro protocol techniques are currently unavailable to help growers to meet the demand of the plant for cultivation and pharmaceutical industry. The present study deals with rapid and efficient protocol development for in vitro propagation of Spilanthes acmella Murr. Shoot induction on Murashige and Skoog medium supplemented with various auxins and cytokinins individually and in various combinations has been achieved by using axillary and apical meristems.MS medium fortified with 1.0 mg/l BAP and 0.1 mg/l NAA was found to be effective individually