Inactivation of Groundnut (Arachis hypogaea L.) Mosaic virus by ultraviolet light
Madhu Mishra
Principal, Anand Vihar College for Women, Link Road no 1. Bhopal 462003 (India) Email: drmadhum@yahoo.com,drbmishra@yahoo.co.in
ABSTRACT
In the present investigation, experiments were conducted in vitro as well as in vivo. In vitro treatment of ultraviolet light clearly indicated that 10 minutes exposure to UV rays had no inhibitory effect on the virus but with an increase in the time of exposure, there was a steady increase in the inhibition of the virus till 55 minutes. At 55 minutes 100% inhibition was noted. In the present study with in vivo treatment, it was observed that except one hour pre-inoculation treatments (inhibition 12.5%), in vivo treatment was not effective. Post-inoculation treatments, in general were more destructive in terms of virus infectivity. Maximum reduction in infectivity was observed in immediate one hour post-inoculation treatment i.e., 87.5% but as the time interval between inoculation and UV exposure increased, there was gradual decrease in virus inhibition.