Phytochemical analysis and TLC Profile of Myristica fragrans Houtt.
Abhilasha Singh1*, Ritu Thakur Bais2 and Vinod Singh1
1Department of Microbiology, Barkatullah University, Bhopal-462026 (India) 2Department of Botany, M.L.B. Girls College, Bhopal-462008 (India)
ABSTRACT
Myristica fragrans Houtt. of Myristicaceae is used in all parts of the world for both as food flavours and for medicinal uses. The present study primarily aims to carry out preliminary phytochemical screenings ,so as to detect the major class of compounds present in the seeds of Myristica fragrans Houtt. and to perform TLC profiling .The extracts were prepared by using hydromethanolic, aqueous and methanol solvents. The yield of extract was calculated for all the three solvents and they were studied for qualitative analysis of phytochemical compounds. Qualitative analysis of the seed extracts confirmed the presence of secondary metabolites such as alkaloids, flavonoids, saponins, tannins, phenols, anthraquinones, cardiac glycosides, and triterpenoids. The methanolics extract of M. fragrans showed highest amount of phytochemicals when compared with other solvent extracts. Thin layer chromatographic studies of the M. fragrans seed extracts constituted different colored phytochemical compounds with different Rf value compound. The present study provides evidence that solvent extract of M. indica contains medicinally important bioactive compounds and this justifies the use of plant species as traditional medicine for treatment of various diseases.