Sharmistha Maity

Department of Botany, Krishnagar Government College, Krishnagar, Nadia-741101 (India) E-mail address: sharmistha_maity@ rediffmail.com; Mobile No. - 9832223933

ABSTRACT

Somatic embryogenesis and plantlet regeneration using embryonic axes of Arachis hypogaea L. variety ICG 6284 had been attempted. Apical portion of the embryonic axes were cultured in Murashige and Skoog’s basal media with various concentrations of 2, 4- dichlorophenoxy acetic acid (2, 4-D). After 30 days of incubation, explants were sub-cultured in the same media without 2, 4-D. Application of N6- benzylaminopurine (BAP) or gibberellic acid (GA3) showed better germination of somatic embryos than the hormone free medium. Survival rate of the in vitro regenerants in the field was above 90%, grew normally and set viable seeds in the experimental garden. Abbreviations: ANOVA- analysis of variance; BAP- N6– benzylaminopurine; DMRT- Duncan’s Multiple Range Test; GA3- Gibberellic acid; MS- Murashige and Skoog’s medium; 2.4-D- 2,4- dichlorophenoxyacetic acid;

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