Roles of Penicillin G and surfactants on extracellular secretion of L-glutamic acid as a function of membrane permeability
Subhadeep Ganguly
Department of Physiology, Vidyasagar College, 39, Sankar Ghosh Lane, Kolkata-700006 (India) *Address for correspondence: res_biol@rediffmail.com
ABSTRACT
The present investigation was undertaken to examine the effect of penicillinG and surfactants on L-glutamic acid production by a biotin auxotroph Corynebacterium glutamicum X680. Addition of penicillin G, 6U/ml, 8h after incubation led to maximum extracellular secretion of Lglutamic acid. Among different fatty acid derivatives (surfactants) studied, Tween60 (polyoxyethylene sorbitan monostearate), 0.5mg/ml, appeared to be the most suitable fatty acid derivative in extracellular secretion of L-glutamic acid from the bacterial cells in presence of biotin when added to the medium after 6h of incubation. Surprisingly, the stimulatory effect was dependent on addition time which was also reflected in the bacterial growth curve of Corynebacterium glutamicum X680.