Emblica officinalis Gaertn. constituents and anti-proliferative activity against an ovarian cancer cell line
Vishal Pundir1* and Aditya Chandell
1Department of Botany, Tilakdhari Post Graduate College, Jaunpur– 222002 Uttar Pradesh (India) Email-vpundir341@gmail.com 2323 Dr. Martin Luther king Jr Blvd New Jersey Institute of Technology, Newark, NJ 07102., USA Email: ac267@njit.edu
ABSTRACT
Emblica officinalis Gaertn. fruits contain structurally diverse phenolic phytochemicals (flavonols, phenolic acids, and hydrolysable tannins) that can exert beneficial effects on the human health. In this report, we describe: 1) methods to process and extract the chemicals from Emblica officinalis juice; 2) qualitative composition analysis of bioactive compounds in Emblica officinalis juice using HPLC-MS-MS techniques; and 3) anti-proliferative activity of the extract against SKOV- 3 cells, a platinum resistant ovarian cancer cell-line. Fresh juice of Emblica officinalis fruits harvested locally in Jaunpur (Uttar Pradesh, India) grown under conventional farming conditions, were crushed with coffee grinder, filtered and concentrated to dry powder to achieve constant weights. HPLC-MS-MS analysis showed the presence of 15 compounds; phenolic acids, and hydrolysable tannins. Treatment with Emblica officinalis extract reduced proliferation of SKOV-3 ovarian cancer cells. In summary, Emblica officinalis fruit juice extract carries a rich profile of phenolic phytochemicals with ant-proliferative activity against a platinum resistant ovarian cancer cell-line.