Polyphenols Contents and Antioxidant Activity of Hertia cheirifolia L. Extracts
Mounira Merghem1* Meriem Hani2 and Saliha Dahamna1
1Laboratory of Phytotherapy Applied to Chronic Diseases, Department of biology and animal physiology, Faculty of Natur and Life Sciences, Ferhat Abbas University, Sétif-1, (Algeria) 2Laboratory of Natural Resource Valorisation, Faculty of Nature Life Sciences, Ferhat Abbas University Setif-1, 19000, (Algeria) Author for Correspondence, mail: mouniramerghem@yahoo.fr
ABSTRACT
Hertia cheirifolia L. is a plant that belongs to the Asteraceae family. It is a medicinal plant traditionally used in the treatment of cramps and stomach pain as well certain gastrointestinal disease. The aim of this study is to evaluate the total polyphenols content and antioxidant activity of the éthanolic extract (EE) and aqueous extract (AqE) of the Hertia Cheirifolia L.. Total phenolic and total flavonoid contents was evaluated by colorimetric method and antioxidant activity by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and β-carotene bleaching assays. The polyphenols and flavonoids contents of ethanolic extract (EE) is 132.395 μg GAE/mg, 11.95 μg QE/mg and aqueous extract (AqE) is 125.2 μg GAE/mg, 8.25 μg QE/mg, respectively. EE and AqE has scavenging activity for DPPH with IC50 0.241±0.007mg/ml and IC50 0.263 ±0.002mg/ml, respectively. In the β-carotene bleaching test, the AqE exhibited the highest antioxidant activity 71.447 ± 7.549% than EE 67.762 ± 8.987%.