Studies on some Haematological parameters including Cellular Phagocytosis interaction occurring in the larval stages as well as in imago of Bombyx mori L. (Lepidoptera: Bombycidae) Breeds
Dipak Kumar Som1*, Somdip Mazumder**, Jesmine Murshed*, Monika Das* and Salil Raha**
*Division of Entomology, Department of Zoology, Maulana Azad College, Kolkata-700013 (India) **Department of Sericulture, Murshidabad University, Berhampore, Murshidabad-700013 (India) 1Corresponding author mail id: dipaksom@gmail.com
ABSTRACT
The motive of the present work is to confer different haematological parameters in healthy larval and adult mulberry silkworm Bombyx mori L. on the three commercial breeds viz. hybrid (Nistari X M12W), bivoltine (NB4D2) and multivoltine (Nistari) along with cellular defensive role in diseased state. Five types of haemocytes, viz. prohaemocytes, plasmatocytes, granulocytes, Spherulocytes, and Oenocytoids are recorded in both healthy adults and 5th instar larvae in contrast to six types of haemocytes including imaginal Spherulocytes recorded prior this study. Discrete Total Haemocyte Count (THC) and Differential Haemocyte Count (DHC) values in the 5th instar larvae among these breeds showed significant differences with the values decreased in the order multivoltine breed, Nistari >bivoltine breed NB4D2. Under infected conditions, THC value in the 5th instar larvae increased in the order of multivoltine breed > bivoltine breed NB4D2 when counted from day 1 to day 4 of developing 5th instar larvae. In both cases declining haemocyte population turn down sharply on the day 5 of 5th instar larval development just prior spinning. Cellular defense response of Nistari breed, against bacterial infection was also studied in their functional aspects, and sequential steps followed in phagocytosis interaction to defend itself against infection from the beginning were also investigated. This comprehensive study may provide an additional reference for the future researches of insect immunity.