In vitro Antiurolithiasis potential of Abrus precatorius L. white seeds
1Gangadhar Bhosale and 2Sumanth Tugaonkar
1Research Scholar, Department of Botany, Mahatma Gandhi Mahavidyalaya, Ahmedpur Latur – 412515 (India) E-mail ID –bhoslegm1991@gmail.com, 2Assistant Professor and Research Guide, Department of Botany, Indira Gandhi Mahavidyalaya, CIDCO Nanded– 431603 (India) E-mail ID – sumanthgt@gmail.com
ABSTRACT
The present research study centered on evaluating In vitro antilithiatic potential of Abrus precatorius L. White seeds. In the present study the seeds of Abrus precatorius have been selected for their antiurolithiatic potential. The plant extract was further subjected to phytochemicals characterizations and found to possess flavonoids, coumarins, saponins, proteins, glycoside, quinones and tannins. In the present investigation, two assays Viz. Crystal Nucleation and Aggregation assay were studied under In vitro conditions in the presence as well as absence of inhibitors. Five different concentrations (100mg/ ml, 200mg/ml, 300mg/ml, 400mg/ml and 500mg/ml) of the plant extracts were tested in each assay. The inhibitory activity of four solvents Viz. Water, Ethanol, Chloroform and Petroleum ether were used for the Calcium oxalate (CaOX) crystal formation, which are principally present in most of the kidney stones. Among all the extracts of Abrus precatorius, the highest percentage of nucleation inhibition (92%) was obtained from water extract at a concentration of 500mg/ml. The aqueous, ethanol and chloroform extracts (100mg/ml to 500 mg/ml) were found to possess significant anti-urolithiatic. The study concludes that the white seed extracts of Abrus precatorius have inhibitory effect on calcium oxalate (CaOX) for crystal nucleation, aggregation and crystal growth. It also found great potential in the dissolution of calcium oxalate crystals. This way, these extracts may be valuable resources for the treatment of urolithiasis.