Girish K1 and Ashok N. Pyati2*

1Department of Microbiology, Government Women’s College, Kolar-563101 (India) 2Plant tissue culture laboratory, Department of PG Botany, Maharani’s Science College for women, Mysore-570005 (India) *Corresponding Author E- mail:


A process of organogenesis via callus with successful plantlet formation was developed for Dendrobium macrostachyum. Indirect organogenesis was achieved from in vitro derived protocorm explants. The explants were cultured on half MS (½ MS) medium fortified with different concentrations and combinations of plant growth regulators such as α-Naphthalene acetic acid (NAA), Benzyl adenine (BA) and Kinetin (Kin). The explants cultured on ½ MS medium supplemented with NAA and Kin alone or in combinations, directly differentiated into the seedlings even after 6 weeks of dark incubation. On the other hand, these explants cultured on ½ MS medium supplemented with BA alone and combination with NAA developed callus after 6 weeks of dark incubation. The optimum callus was developed in BA (4.44μM) and Combination of BA (4.44μM) with NAA (1.08 μM) induced 36.4% and 59.9% of callus respectively. This callus on subculture to the same media developed protocorm like bodies (PLBs). These PLBs later differentiated into healthy plantlets. A survival rate of 90% under greenhouse conditions was achieved.

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