Comparison and analysis of IgE and blood histamine levels: its correlation during allergic sensitization in atopic and non-atopic population
a,bHemavathi A., a,bD.P. Shruthi Shree, cP. Niranjana, dParisara Nagaraj, bBettadatunga T. Prabhakar and aSiddanakoppalu N. Pramod1*
aFood Allergy and Immunology Lab, Department of Studies in Food Technology, Davangere University, Shivagangotri, Davangere-577 007, Karnataka (India) bBiomedicne Lab, Department of Biotechnology, Sahyadri Science College, Kuvempu University, Shimoga-577203 (India) cDepartment of Biochemistry, Kuvempu University, Shankaragatta-577451, Shimoga, Karnataka, India. dDepartment of Environmental Sciences, Sahyadri Science College, Kuvempu University, Shimoga-577203, Karnataka, (India) *Correspondence address Dr. Siddanakoppalu N. Pramod Food Allergy and Immunology Lab, Department of Studies in Food Technology, Davangere University, Shivagangotri, Davangere-577 007, Karnataka, (India) Mail: snpramod2029@gmail.com, Phone: +91-9148356589
ABSTRACT
Independent and inter-dependent troupe of histamine- an inflammatory immune mediator together with Immunoglobulin E (IgE) and mast cells shoulder the responsibility of clinical allergic sensitization in atopic population which ends-up in the severe anaphylactic and acute allergic reactions. Histamine with their receptors (H1R-H4R) plays a significant managerial role in several allergic diseases. In this study an emphatic effort has been employed to screen, evaluate and compare to understand the serum and plasma histamine levels in atopic and nonatopic populations and in turn correlated with total IgE levels to understand interrelation of IgE with histamine content during allergic reactions. The atopic and non-atopic subjects were selected based on subjective case histories and were analysed for blood Eosinophilic count, Total IgE levels and plasma and serum histamine levels. Leukocyte isolation, rat peritoneal exudate mast cells isolation and histamine release quantification and their HPLC screening, finally correlating the total IgE with Histamine released were assayed by comparing the values obtained with atopic, non-atopic samples and with known standard values of histamines, leukocytes, eosinophil’s of Normal healthy people.