Effect of TDZ on callus induction and estimation of Phenol, Flavoniod and Antioxidant property in in vivo and in vitro grown Centella asiatica (L.) Urban
Shweta Kumari and Maheshwar Prasad Trivedi
Department of Botany, Patna University, Patna-800005 (India) Email Id- shwetabt21@gmail.com, mptrivedi1956@rediffmail.com Shweta Kumari ORCID ID – 0000-0001-8823-4431
ABSTRACT
Medicinal plants are enriched in various bioactive molecules such as vitamins, terpenoids, phenolics, lignins, stilbenes, tannins, flavonoids, quinines, coumarins, alkaloids, amines, betalains and other metabolites. The present study was carried out to establish an efficient and reproducible protocol for callus induction in Centella asiatica (L.) Urban belonging to family Apiaceae. Combination and composition of different plant growth regulators were used for callus induction. Out of various compositions MS medium fortified with TDZ 1mg/l and NAA 0.1 mg/l showed maximum response in both stem and leaf explants in terms of callus induction. Although MS medium supplemented with BAP 4.5 mg/l and NAA 0.1 mg/l also showed better responses. TDZ was the best for callus induction (91.6%) of callus against BAP respectively (75 %). Stem showed better explants (83.3 %) for callus induction as compared to leaf explants (75 %). Determination of phenolics and flavonoid contents were performed by using Folin-Ciocalteu reagent and aluminium chloride colorimetric method. Free radical scavenging activity was estimated by DPPH (1, 1-diphenyl-2-picrylhydrazyl). Maximum phenolics (6.35) and flavonoid contents (37.5) were obtained from leaf derived callus. Antioxidant property was also highest in leaf derived callus. Micropropagation allows conservation of this plant and can be used to produces large scale of clones in a continuous manner and callus induction enhances production of bioactive molecules.