*Aman Kumar, Deepshikha Samdershi, Uzma Shamim and Priti Kumari

University Department of Zoology, Ranchi University, Ranchi-834008 (India) *Corresponding author- Email id- amankumar007official@gmail.com


Recent trend of using hybrid pesticides in agricultural practices have drawn the attention of researchers to investigate their cumulative effects on the non- target organisms, especially fish. In the present study, toxic effects of hybrid pesticide containing chlorpyrifos (50%) and cypermethrin (5% EC) on liver of Clarias batrachus have been investigated. Healthy fish of 20-25 cm length and 75-100 g weight were purchased from local fish market in Ranchi and were kept for acclimatization in laboratory conditions for two weeks. The fish were divided into 3 groups (n= 15). Group I served as normal control and group II (LD) and III (HD) were treated with sub-lethal concentrations of 1/15th and 1/5th of LC 50, respectively. After 96 h, fish from each group were weighed and sacrificed under anesthesia. Liver tissues were dissected out, weighed and processed for preparation of histological slides stained with H & E. Hepatic tissues were further studied for changes in hepatosomatic index and histopathological alterations. Data were statistically analyzed using Student’s t- test. Acute exposure with hybrid pesticide significantly increased the HSI in high- dose group fish (p<0.05). Treatment with hybrid pesticide resulted in mild to moderate alterations in hepatic tissues in a dose dependent manner, such as, loosening of hepatic parenchyma, cytoplasmic and nuclear degeneration, hypertrophy of hepatocytes, fatty accumulation, sinusoidal alterations, activation of Kupffer cells, infiltration with leucocytes, congestion and disruption in central vein, hemorrhage etc. On account of hepatic toxicity induced by hybrid pesticide, effective control measures for its use and disposal should be adopted.

Key words : Chlorpyrifos, Cypermethrin, Hybrid, Clarias, batrachus, Hepatocytes, Acute.

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