Callus induction in Carica papaya from hypocotyl and leaf explants
1Shweta Kumari, 2Ajeet Kumar and 3Nitish Kumar
1,2Department of Botany, Patna University, Patna-800005 (India) Email Id- shwetabt21@gmail.com, iajeetji@gmail.com 3Department of Biotechnology, Central University of South Bihar, Gaya-824236, Bihar, India Shweta Kumari ORCID ID – 0000-0001-8823-4431
ABSTRACT
The papaya is fruit of the plant Carica papaya, the sole species in the genus Caricaof the plant family Caricaceae. It is a source for proteolytic enzymes papain and chymopapain. The crop improvement programmes of papaya are faced with problems due to its heterozygosity, dioceous nature. Therefore, the present investigation focused on to standardize a protocol for rapid callus induction in Carica papaya species (Honey Dew var.). Juvenile hypocotyls, leaf and mature midrib were cultured on full strength MS medium containing a range of Kn, NAA, 2,4-D and BAP with different composition. Hypocotyl and leaf formed maximum callus on medium containing 1 mg/l Kn followed by 0.25 mg/l NAA and Kn 5 mg/l fortified with NAA 0.25 mg/l respectively. Hypocotyl and leaf explants proved to be the best explants for callus induction. Midrib unresponsive towards the callus induction. Kinetin proved better growth hormone than BAP. The method may be useful for the mass propagation of female papaya plants for large scale cultivation.