Isolation and characterization of cultivable ndophytic micro biome associated with wild fern Azolla
1Varsha Savaner and 2*Deepak Sinha
1Institute of Biological Science, Sage University, Indore-452020 (India) 2Institute of Agriculture, Sage University, Indore-452020 (India) 1 varshasavaner@gmail.com , 2* Corresponding author: drdeepaksinhasage@gmail.
ABSTRACT
Endophyte constitute the important part of plant microbiome have been shown to benefit plant health in a variety of ways. The present investigation was aimed to isolate and characterize endophytic bacteria from wild fern Azolla. Within this study total of 15 endophytic bacteria were isolated from Azolla by standard microbiological culture methods. Based on 16S rDNA sequences, the isolated species comprising bacteria commonly associated with soil and plants. The genera Bacillus polymyxa, Bacillus thuringiensis, Proteus mirabilis and Azotobacter species were isolated from fern. All isolates were screened for the production of enzymes. The result of the study revealed that maximum isolates have positive protease, urease and amylase activity and this was followed cellulase activities. The result of the present study indicated that among the above four novel strains of endophytic bacterial isolates Bacillus thuringiensis and Bacillus polymyxa has wide range of agriculture application and potential biological control agents, Future research will analyse that these isolates can be used for biological control, growth promotion, medical application or the synthesis of enzymes for biotechnology.