Madhuca longifolia ethanolic seeds extract attenuates chemical carcinogenesis-induced oxidative injury through antioxidation and inhibition of DNA damage to skin and liver cancer in Swiss albino female mice model
1*Dibyajyoti Saha and 1Satish Kumar Sarankar
1Faculty of Pharmacy, Mansarovar Global University, Billkisganj, Sehore-466001 (India) *Corresponding Author: djsmgupharmacy@gmail.com
ABSTRACT
Epidemiological and experimental evidence suggested that phytochemicals have potent cancer chemopreventive agent and also active against skin and liver cancer. Exhaustive studies revealed that the Madhuca longifolia have more potential as chemopreventive agents due to their lower toxicity. DMBA known as tumor initiator and croton oil is a highly active tumor promoter. The purpose of the research was to explore the chemopreventive activity of Madhuca longifolia ethanolic seeds extract in a 7,12 dimethylbenz (a) anthracene (DMBA)-croton oil carcinogenesis model. Five groups were designed for the study and Swiss albino female mice were intraperitoneally injected with vincristine 50mg/kg b.w. at a standardized dose. Mahua extract was given orally at an optimum dose of 15 mg/kg b.w. and 30 mg/kg b.w. as per experimental protocol. The parameters LPO, GST, SOD, CAT, GSH have been evaluated. The results obtained showed significant elevation of glutathione-s–transferase, superoxide dismutase, catalase, reduced glutathione and inhibition of lipid peroxidation in skin and liver. The results obtained showed significant reduction of the number of skin papillomas along with elevation of detoxifying enzymes and inhibition of lipid peroxidation in the skin. The results established that the plant seeds have potent skin and liver cancer chemopreventive agent. Further studies need to be done to understand the mechanism of action.