Isolation, screening and Biochemical identification of plant growth promoting Rhizobacteria of Lablab purpureus (L.) Sweet
1Aarti, 2Vijay Pal, 1Shivam Parmar, 1Sujata and *Anil Kumar
1Department of Botany, Raja Balwant Singh College, Dr. Bhimrao Ambedkar University, Agra-282002 (India) 2Amity Institute of Biotechnology, Amity University Rajasthan, Jaipur-300202 (India) *Pricipal, Maharaj Singh Degree College, Ch. Charan Singh University, Meerut-247001 (India) *E-mail: asharma2341@gmail.com; Mobile: 8168122659
ABSTRACT
In the current study, rhizospheric soil and nodules from Indian bean are used to isolate, a total of twelve colonies of distinct rhizobacteria on YEMA media, through serial dilution method. Soil samples were collected from nine randomly selected healthy Dolichos bean plants containing root nodules and root-adhered soil. A total of twelve isolated bacteria were screened in vitro for different plant growth promotion activities i.e. phosphate solublization, IAA production, siderophore production, ammonia production and biochemical testing. In the present work out of twelve bacterial isolates six was positive for phosphate solublization. IAA production and siderophore production was shown by eleven bacterial isolates. All isolates were positive for ammonia production, starch hydrolysis, citrate and catalase test and further identified on the basis of colony morphology, Gram staining and biochemical tests. These isolates were identified as Escherichia. coli, Bacillus sp. As PGPR are environmental friendly and offer sustainable approach to increase production of crops and health. Therefore, these isolates can be utilized for biofertilizer formulation under local agroclimatic conditions.