Isolation, molecular characterization and evaluation of efficient Azotobacter on Sorghum plant
1*Bushra Khader Ustaad, 1Dayanand Agsar, 2Mahadevaswamy and 1Sirasagikar Reshma N
1A-DBT Research Laboratory, Department of Microbiology, Gulbarga University, Kalaburagi-585106 (India) 2Department of Agricultural Microbiology, College of Agriculture, University of Agricultural Sciences, Raichur-584101 (India) 1*Correspondence author email: bushraustaadm@gmail.com
ABSTRACT
Fly ash is a rich source of essential plant nutrients hence it is suited for crop production and thus facilitates alternate use for fly ash. In the present investigation, 99 Azotobacter isolates were isolated from the rhizospheric soil from cereal fields, All the isolates were morphologically, biochemically characterised. Isolates were screened to obtain efficient Azotobacter isolates. The Azotobacter isolate BUDA2 fixed highest amount of nitrogen 13.3g-1 of mannitol. Based on 16s rRna analysis of an isolate BUDA2 was identified as Azotobacter chroococcum. A pot culture experiment was conducted to study the effect of BUDA2, fly ash and RDF on the growth parameters of sorghum plant. The inoculation of BUDA2 along with 10% fly ash and 75% RDF showed significant increase in growth parameters of sorghum.