Induction of callus from lesser duckweed {Lemna minor L.} on Basal media fortified with PGRs.
Sudipta Kumar Sil1 and Santanu Gupta*
1Department of Botany, University of Gour Banga, Malda-732103 (India) *Department of Botany, Malda College, Malda-732101 (India) *Corresponding Author: Email : santanubotanist@gmail.com
ABSTRACT
Lemna minor L. is gregariously growing aquatic macrophyte which can be propagated in vitro in liquid basal media. The current paper focuses on callus induction from aseptic fronds of Lemna minor L. in three different types of basal culture media namely Murashige & Skoog basal medium; Gamborg B5 basal medium; SH- Schenk & Hildebrand basal medium supplemented with three PGRs namely IBA- {Indole butyric acid}; 2, 4- D {2,4-dichlorophenoxy acetic acid}; BAP- {6-benzyl aminopurine}. MS medium fortified with PGRs was least responsive in callus initiation whereas Gamborg B5 medium and SH media supplemented with IBA and BA were most effective in callus induction from fronds of Lemna minor L.