Isolation and identification of biosurfactant- bacteria from diverse oil samples and their application
1Fathimath Farseena and 2Achuth Jayakrishnan
1,*2Department of Microbiology, Hindusthan College of arts and science, Coimbatore - 641028 (India) *2Corresponding author- achuaj08@gmail.com
ABSTRACT
Biosurfactants are surface active components produced by various microorganisms as their extracellular or cell wall-associated compounds. And these biosurfactants have future promising properties in various fields because of their biodegradability, low toxicity, good environmental compatibility, high foaming property, high selectivity, and specificity at extreme environmental conditions. The present work aims to isolate and characterize biosurfactant-producing bacteria from oil samples {battery, engine, and crude oil} and evaluate their antimicrobial and biofilm inhibition potential against different pathogens. Samples were collected from different locations in Coimbatore and the bacterial isolates were screened for production of biosurfactants. Biosurfactant activity evaluation was carried out by oil displacement, drop collapse test, oil spreading assay, emulsification assay, and hemolytic assay. 16s rRNA sequence analysis was used for the isolate identification and the isolates were determined as Metabacillus schmidteae, Bacillus sp. {in firmicutes}, and Pseudomonas aeruginosa. The same was deposited in the GenBank repository with accession numbers OQ735442.1 {Metabacillus schmidteae}, OQ745826.1 {Bacillus sp. {in firmicutes}} and OQ746451.1 {Pseudomonas aeruginosa}. The biofilm inhibition assay was determined against Staphylococcus aureus and Klebsiella pneumoniae, wherein the biosurfactants from all three isolates showed promising potential in comparison with the positive control