Isolation and characterization of indigenous Bacteria from contaminated soil samples of Kamrup District, Assam
*Dhritiman Chanda, 1G.D. Sharma and Rakkame G. Momin
Dhritiman Chanda Department of Botany, University of Science& Technology Meghalaya-793101 India *Corresponding author, Email Id:dhriti.delhi@gmail.com
ABSTRACT
Soil contains a various number of microorganisms in it. Some of these microorganisms that reside in soil are harmful, while some are useful. The present investigation was carried out to isolate and characterization of bacteria from polluted soil samples. Selected bacterial isolates were evaluated by morphological, and biochemical test. Gram staining of the bacterial isolates was also performed. Based on Gram’s staining, 5 bacterial isolates were Gram Positive, while the remaining 3 isolates were Gram Negative. According to Biochemical test (Urease test, Starch Hydrolysis test, Catalase test) done on isolates, Isolate 3 and Isolate 6 showed Negative on all the tests, while Isolate 7 showed positive result only on catalase test. Isolate 1, Isolate 2, Isolate 4, and Isolate 5 showed negative result only on Urease test. While Isolate 8 showed negative result only on catalase test. Out of eight selected bacterial isolates, only Isolate 8 showed positive for Urease test. Antibiotic sensitivity test also showed significant result for the selected isolates. The isolated bacterial strains will be helpful for possible application of bioremediation of contaminated sites.