A study on cytotoxic and anticytotoxic effects on Allium cepa L. root tips treated with Gamma irradiated Amaranthus dubius Mart. ex. Thell. aqueous seed extracts
1N. Priyanka and 2V. Manimozhi
Department of Plant Biology and Plant Biotechnology Ethiraj College for Women -Autonomous, Chennai – 600 008 India Corresponding author:vmtv2018@gmail.com
ABSTRACT
The present investigation appertains about the Amaranthus dubius Mart. Ex Thell. Var (Co1) dried seeds exposure under gamma radiation at different doses (50,100,150 & 200 Gray) and the effect of gamma irradiated seed powder on somatic chromosomes of Allium cepa L. The seed powder was taken at various concentrations such as 5%, 7% & 9%. For each concentration, the treatment time has been confined to 12hrs, 18hrs and 24hrs respectively. Different types of chromosomal aberrations such as chromosomal fragments, chromosomal bridges, stickiness, binucleate cells and laggards has been observed frequently in the treated root tips of Allium cepa L. The onion root tip assay has been used to determine the mitotic index, abnormalities in the chromosomes and hindrances in the mitotic cell cycle. Thus the present work concludes that the acute and chronic effects of gamma radiation on Amaranthus dubius seeds consequently targeted the mitosis index and chromosomal aberrations of the A. cepa L. cells,depending on potency and time span.