Green synthesis of silver nanoparticles using Andrographis paniculata (Burm. f.) Nees stem extract and their evaluation for anti-cancer activity
1 Rahul Meshram, 2 Rabecca Kapure and 2* Utpal Dongre
1Department of Biochemistry, Sindhu Mahavidyalaya, Panchpaoli, Nagpur - 440017 India 2Department of Biochemistry and Biotechnology, Dr Ambedkar College, Deekshabhoomi, Nagpur - 440010 India Corresponding author*: Dr Utpal Dongre, utpal24dongre@gmail.com , 9730938751
ABSTRACT
Cancer is often characterised by uncontrolled cell growth due to loss in cell death mechanisms. It is emerging as a global pandemic affecting huge number of people across the globe. Design and development of new anticancer agents using conventional methods are always challengeable due to their life-threatening side effects. These limitations may overcome by the use and green method synthesised nanomaterials. But to impart their effects in biological system by retaining in intracellular environment a proper shape and size of nanoparticle is essential. Regarding the same, present study aims to synthesise silver metal nanoparticles using Andrographis paniculata stem extract and to evaluate their anti-lung cancer activity. Material characterisation were done through Scanning Electron Microscopy {SEM}, Energy dispersive X Ray analysis {EDAX}, Transmission Electron microscopy {TEM} and Fourier transform infrared {FTIR} spectroscopy analysis. EDAX and SEM analysis showed presence of silver nanoparticles in synthesised material with rough spherical morphology respectively. TEM analysis showed nanoparticles with size ranged from 13-27 nm. Decreased A549 cell viability was reported when cells were treated with synthesised silver nanoparticles.