Study of morphological and Biochemical characterization of Rhizobium isolated from Root nodules of Cajanus cajan L. exposed to Cement dust pollution
*1Renuka Siddanna, 2Sudarshan Ashok and 3Dayanand Agsar
*1Department of Environmental Sciences, Gulbarga University, Kalaburagi-585106 {India} 2,3A-DBT Research Laboratory, Department of Microbiology, Gulbarga University, Kalaburagi-585106 {India} *1Corresponding Author: renukastalwar@gmail.com Contact number: 8310302070
ABSTRACT
In the present study, 11 rhizobium strains were isolated from root nodules of redgram {Cajanus cajan} crop which is exposed to cement dust of Kalaburagi cement industry of sedam taluk and Chettinad cement industry of Chincholi taluk and control field is far away from these two cement industries. Rhizobium isolates were isolated using selective media such as Yeast Extract Mannitol Agar media {YEMA}. The isolates were identified as rhizobium on the basis of their morphological, biochemical and confirmatory tests. All isolates were gram-negative, rod shaped and poor absorption of red color when cultured in YEMA containing Congo red. In the ketolactose test yellowish zone of Cu2O not found. All isolates showed fast growth in YEMA+BTB. Based on the Morphocultural and Biochemical studies, results were confirmed that the isolated strains were rhizobium.