Somatic embryogenesis and quantification of L-Dopa in Mucuna pruriens (L.) DC - A multipurpose medicinal legume
Motilal Srivastava, Aleya Siddiquee, Rajeev R Baitha and *Md. Sarfaraz Ahmad
University Department of Botany, Jai Prakash University, Chapra - 841301 India Corresponding author: *mdsarfarazahmad786@gmail.com
ABSTRACT
Secondary metabolite production can be achieved by using Plant cell culture method as a choice. The plant Mucuna pruriens L. DC an annual legume is rich in an exceptionally important amino acid called L-Dopa, 3-3, 4-dihydroxyphenyl - L-alanine. The present study reports the somatic embryogenesis induction in Mucuna pruriens L. DC. The study includes the collection of nodal and internodal samples from the mature plant, the callus with the best features was carried forward for the somatic embryogenesis process followed by its germination in both solid and liquid media. The well propagative somatic embryos were then taken forward for acclimatization and finally transferred to the fields. The plants were then analyzed for the presence and quantitative analysis of L-Dopa in them via extraction from tissue and quantification by HPLC method. The conclusion driven from the study is that the somatic embryogenesis is a better way for large scale and high yielding propagation of Mucuna pruriens L. DC as wells as it yields the plantlets with good L-Dopa concentration, which is a pharmacologically important compound