ICPOES Based seed micronutrient content analysis as Tool to identify Allelic Sergeants’ in a Stable RIL mapping population of Foxtail millet (Setaria italica L.)
1# Shaik Sameena, 1#Anand Kumar, 1 Konda Sravan Simha Reddy, 1 Sake Ananda Raja Kumar, 1Gurulakshmi Kola, 1Mallikarjuna Gunti, 2Puli Chandra Obul Reddy, 3C. V. C. M. Reddy and *1A. Chandra Sekhar
1Molecular Genetics and Functional Genomics Laboratory, Department of Biotechnology, School of Life Sciences, Yogi Vemana University, Kadapa-516005 India *chandrasekhar9@yahoo.com and chandrasekhar9@yvu.edu.in #Equal Contribution 2Plant Molecular Biology Laboratory, Department of Botany, School of Life Sciences, Yogi Vemana University, Kadapa-516005 India 3Principal Scientist - Planning & Monitoring Cell, Administrative Office, ANGRAU, Lam, Guntur - 522 034 India
ABSTRACT
Foxtail millet Setaria italica L. is a small grained C4 Panicoid cereal and a family member of Poaceae, with a small genome size of about ~515 MB. It serves as staple food in dominant arid and semi-arid regions of Asia and Africa. Being a fact that millets are nutritionally superior, rapid urbanization and green revolution has contributed for substantial shrinkage of millet cultivable area. Reduced freshwater, fertile lands and unpredicted environmental factors has led to the comeback of the millets as an answer to global Nutri-food security challenges. Based on earlier work from our lab, genetically distinct genotypes for seed micronutrients and yield related traits were used as parental lines for stable RIL mapping development. Testing the hypothesis of segregating alleles in the population, randomly selected RIL’s along with their parental lines were subjected to ICP-OES analysis. High genetic variability for the seed micronutrient content with respect to iron, zinc, copper and manganese has been observed in the RIL’s. Descriptive statistics and frequency distribution reveals the high micronutrient contents in developed RIL population when compared with their parental lines. The results are evident of the segregating genetic determinants controlling seed micronutrient content that can be used for breeding foxtail millet for enhanced micronutrient content.