Studies on phytochemicals and antibacterial potential of Apluda mutica L. : An underutilized ethnomedicinal grass
C. N Shruthi and *D Kotresha
Department of Studies in Botany, Davangere University, Shivagangothri, Tholahunase, Davangere-577007 (India) *Correspondence: Kotresha D. Department of Studies in Botany, Davangere University, Shivagangothri, Tholahunase, Davanagere-577007 (India) Email: dkotresh@davangereuniversity.ac.in
ABSTRACT
Apluda mutica L. is an underutilized ethnomedicinal grass species that belongs to the Poaceae family. Ethnomedicinallyit has been used to treat mouth sores in cattle and dysentery. The main objective of the current research work is to identify the bioactive constituents and in vitro antibacterial potential. The qualitative biochemical profiling revealed the presence of alkaloids, steroids, flavonoids, terpenoids, tannins, phenols, glycosides, proteins, and carbohydrates. The LCMS profiling of whole plant methanolic extract showed the existence of 10 bioactive compounds. 2-O-rhamnosyl-swertisin (45.00 %); Sennoside C (27.32 %); Renchangianin B (7.13 %) andQuercetin 3,7-dimethyl ether (6.16 %) were the major identified compounds. the antibacterial activity was evaluated by using an agar well diffusion assay and the results revealed that the methanolic whole plant extract showed moderate antibacterial activityand exhibited the highest inhibition activityagainst Enterococcus faecalis (12.16±0.44 mm), followed by Staphylococcus aureus (10.66±0.66 mm) and Escherichia coli (07.16±0.44 mm) and the minimum zone of inhibition was showed against Pseudomonas aeruginosa (04.66±0.66 mm). Further in vivo confirmations are needed to prove the real antibacterial and anticancer efficacy of the test plant Apluda mutica.