Role of Nitric Oxide in Stress mitigation in Fenugreek seedlings during the Post-germination Phase under Salinity stress
*1Saran Kumar Gupta, 2Suchisree Jha and 3Palash Mandal
1Department of Botany, Kalimpong College, Rinkinpong Road, Kalimpong – 734 301 India 2Plant Nutrition Department, Indofil Industry Ltd., Sandoz Baug, Thane West, Maharashtra – 400607 India 3Plant Physiology & Pharmacognosy Laboratory, Department of Botany, University of North Bengal, Darjiling – 734013 India *Correspondence: nbubotanysaran@gmail.com
ABSTRACT
In the present study the exogenous sources of nitric oxide (SNP) and its scavenger (c-PTIO) were applied for studying their effect on the alteration of various biochemical parameters along with their antioxidant defence system during germination stages. The pre-treated seeds were exposed to saline condition and their various morphological, physiological and biochemical attributes were determined. Our results suggest that priming of fenugreek seeds with exogenous source of nitric oxide enhanced the morphological and biochemical attributes along with the antioxidant defence system under saline condition, which was further substantiated by the occurrence of adverse effects of salinity on the seeds which were unprimed and also those primed with c-PTIO, a nitric oxide scavenger. Therefore, the enhancement in the enzymatic as well as non-enzymatic components might be due to the involvement of nitric oxide leading to tolerance towards salinity accompanied with better growth and development. Additionally, an uninterrupted influx of these signal molecules is very much essential for better growth and salinity stress management in fenugreek seedlings, which can be hypothesized on the basis of the tremendous deterioration of the growth parameters as well as oxidative stress management status of the fenugreek seedlings revealed in the present study.