Study of the antimicrobial activity of the solvent extracts of mangrove plants associated Bacillus species
Ruchi Malik and Rajesh Patil
Department of Microbiology, Bhartiya Vidya Bhavan’s M.M College of Arts & N.M Institute of Science & H.R.J College of Commerce (Bhavan’s College) Munshi Nagar, Near Azad nagar metro station, Andheri west, Mumbai – 400058 (India) Phone No: +919920966515 Email Id: ph.dscholarruchi02@gmail.com Email Id: rcpatil68@gmail.com
ABSTRACT
Mangrove associated bacterial species are the major inhabitants that represent a rich reservoir for production of bioactive compounds. The isolates procured from the mangrove environment were enriched and screened for potent compounds using agar strip method, agar streak method and Wilkin’s overlay method. The isolates showing inhibition against the test pathogens were subjected to identification using partial 16s rRNA gene sequencing, which were found to be Bacillus species. Isolates were bulk seeded into the nutrient medium, grown, followed by removal of bacterial cellsusing membranefiltration technique. Thefiltrate was mixed with equal amounts of solvent and kept overnight under shaken conditions. The two layers were separated using a separating funnel. The solvent layer was concentrated and its efficacy was checked against various pathogens like Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Proteus mirabilia, Klebsiella pneumoniae and Salmonella typhi using agar cup method. The solvent concentrates showed inhibition against the pathogens which confirmed the antimicrobial activity of Bacillus species.