Butanolic fraction of Butea monosperma ameliorates inflammation and angiogenesis in Chick chorioallantoic membrane
1*R. L. Shirole, N.L. Shirole, and M. N. Saraf3
1Department of Pharmacology, DCS’s A. R. A. College of Pharmacy, Dhule - 424005 {India} Email id: rahulshirole@gmail.comTel: 02562-2243021, Orcid ID: https://orcid.org/0000-0002-9410-6097 2Department of Pharmaceutical Chemistry, DCS’s A. R. A. College of Pharmacy, Dhule - 424005 {India} Email id: nitinshirole@gmail.comTel: 02562-2243021, Orcid ID: https://orcid.org/0009-0002-2565-6201 3Department of Pharmacology, H.K. College of Pharmacy, Mumbai - 400102 {India} Madhusudansaraf@gmail.com *Corresponding author Dr. R. L. Shirole {M. Pharm., Ph.D.} Associate Professor of Pharmacology, Dept. of Pharmacology, DCS’s A. R. A. College of Pharmacy, Dhule - 424005 {India} Email id: rahulshirole@gmail.comTel: 02562-2243021
ABSTRACT
The present investigation aimed to elucidate the probable mechanism of underlying anti-inflammatory and antiangiogenic action of Butea monosperma. To obtain the ethyl acetate and butanolic fractions ofButea monosperma, bioactive fractionations of the methanolic extract of the plant were performed. Since BBM had the most phytoconstituents according to HPTLC characterization, we have decided to investigate it further. The impact ofButea monosperma’s butanolic fraction was evaluated on the erythropoietin-induced angiogenesis on the Hen’s Egg Test Chorio-Allantoic Membrane Assay {HET-CAM Assay} and the acute angiogenic response on the SDS-induced acute inflammatory response. Butanolic fraction of Butea monosperma {25µg/mL, 50 µg/mL and 100 µg/mL} SDS- induced inflammation in HET CAM Model. Similarly on erythropoietin- induced angiogenesis it exhibited significant inhibition {p < 0.0001} of angiogenesis dosedependently {25µg/mL, 50 µg/mL and 100 µg/mL}. In the present investigation the Butanolic fraction of Butea monosperma exhibited anti-inflammatory and anti-angiogenic action on chick chorioallantoic membrane.