Estimation of oxidative stress in type 2 Diabetes mellitus patients in correlation with hbaic
S. Hari Priya1*, Nagendra Babu2, Praveen Kumar3 and Triveni4
1*,2,3,4Department of Biochemistry, School of Allied Healthcare and Science, Malla Reddy University, Hyderabad - 500055 (India) 1*Corresponding author: Dr. S. Hari Priya Mail ID: drharipriya@mallareddyuniversity.ac.in 1*Associate Professor, 2Professor, 3Assistant Professor, 4Assistant Professor
ABSTRACT
Diabetes mellitus {DM} is a multi factorial disorder that occurs when the body either does not produce enough insulin or cannot effectively use. Oxidative stress, which results from an imbalance between the production and elimination of free radicals, is a major contributor to poor glycemic control and complications in individuals with diabetes. To evaluate the level of oxidative stress in patients with type 2 diabetes mellitus [type 2DM] compared to healthy individuals. The study included a total of 120 participants, with 60 diagnosed with type 2 diabetes [test group] and 60 healthy individuals [control]. Approval was obtained from the institutional ethical committee followed by venous blood sample was collected from diabetic and healthy subjects to measure fasting blood sugar [FBS mgperdl], postprandial blood sugar [PPBS mgperdl], HbA1c [%], total antioxidant capacity [TAC mmolperdl], and malondialdehyde [MDA mmolperdl]. The data obtained was analyzed using SPSS software version 23.0. The results showed that diabetic patients had higher levels of HbA1c, FBS, PPBS, and MDA and lower levels of TAC compared to healthy subjects. The differences in these variables between the two groups were statistically significant. Patients with type 2DM exhibited elevated blood glucose levels [HbA1c, FBS, and PPBS] and MDA, along with decreased TAC, indicating an increase in oxidative stress. Furthermore, a strong negative correlation was observed between HbA1c and TAC in the diabetic group, suggesting that as blood glucose levels rise, oxidative stress also increases.