Formulation of Novel Culture medium for the cultivation of Biosurfactant producing Bacteria using Agrowastes and Crop residues
A. Sudha, P. Saranraj*, T. M. Sadiqua Jabeen and M. S. Swetha
PG and Research Department of Microbiology, Sacred Heart College (Autonomous), Tirupattur - 635601 (India) *Corresponding Author E.mail: microsaranraj@gmail.com
ABSTRACT
The present research was aimed to formulate the Novel culture medium for the Biosurfactant producing microorganisms like Bacillus subtilis [Surfactin, Subtisin, Iturnin and Fengycin], Pseudomonas fluorescens [Rhamnolipids, Viscosin andArthrofactin], Achromobacter sp. [Iturin] and Rhodococcus sp. [Rhamnolipids]. The Biosurfactant producing bacteria in Soil samples was isolated in the Nutrient agar plates by Serial Dilution Method [Pour plate method] and, identified by Microscopic examination [Gram staining, Endospore staining and Motilitytest], Platting in Selective medium and Biochemical tests. The isolated bacterial strains were screened for its ability to produce Biosurfactants, all the isolated bacterial strains have an efficiency to produce Biosurfactants. The Biosurfactant producers were cultivated in Agrowastes [Orange peel, Lemon peel, Banana peel, Sweet lime peel, Sugarcane bagasse, Cassava peel and Potato peel] and Crop residues [Rice bran] for lowering the cost ofcultivation medium. In Solid medium, all the Biosurfactants producing bacteria showed good and luxuriant growth. In Liquid medium, Biosurfactants producing bacteria showed good growth in the form of Turbidity in the Liquid medium. In conclusion, utilization of Agrowastes and Crop residues for the Biosurfactant production reduces the waste accumulation in the environment and increases the Biosurfactant yield.