1Prashant Singh, and 2Neeraj Kumar Dubey

Department of Botany Rashtriya P. G. College Jamuhai, Jaunpur - 222001 (India)

ABSTRACT

A key component of integrated nutrient management, sustainable agricultural productivity, and environmental preservation is the use of biological nitrogen fixation (BNF). The main aim of the study is to inoculating sugarcane micro propagated with Gluconacetobacter diazotrophicus and Herbaspirillum sp., we were able to save n-fertilizer. When these two strains of bacteria were mixed together, the short-term experiment showed a significant increase in biomass under N limited conditions. A decrease in the population of was seen throughout the same time period, but the population of inoculated Herbaspirillum sp. remained consistent during the long-term experiment. When plants were infected with G. diazotrophicus and Herbaspirillum sp. and then fertilised with 50 percent of the prescribed N (140 kg ha-1), the total biomass and leaf N content were greater than when the recommended quantity of inorganic N was used on the plants that weren’t (280 kg ha-1).

Key words : Inoculating, Sugarcane, Micropropagated, Gluconacetobacter diazotrophicus, Herbaspirillum sp.

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